[Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].
Identifieur interne : 002C40 ( Main/Exploration ); précédent : 002C39; suivant : 002C41[Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].
Auteurs : Khayir Yusuf [République populaire de Chine] ; Shirenay Pulat ; Maripat Turdahun ; Maryam Hamdun ; Ghenijan Osman ; Zumrat Abdurehim ; Erkin RahmanSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2011.
Descripteurs français
- KwdFr :
- Bactéries (classification), Bactéries (génétique), Bactéries (isolement et purification), Biodiversité (MeSH), Chine (MeSH), Données de séquences moléculaires (MeSH), Phylogenèse (MeSH), Phénomènes physiologiques bactériens (MeSH), Populus (microbiologie), Populus (physiologie), Rivières (microbiologie), Symbiose (MeSH).
- MESH :
- génétique : Bactéries.
- isolement et purification : Bactéries.
- microbiologie : Populus, Rivières.
- physiologie : Populus.
- Bactéries, Biodiversité, Chine, Données de séquences moléculaires, Phylogenèse, Phénomènes physiologiques bactériens, Symbiose.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- MESH :
- geographic : China.
- classification : Bacteria.
- genetics : Bacteria.
- isolation & purification : Bacteria.
- microbiology : Populus, Rivers.
- physiology : Populus.
- Bacterial Physiological Phenomena, Biodiversity, Molecular Sequence Data, Phylogeny, Symbiosis.
Abstract
OBJECTIVE
The aim of this study was to identify the culturable endophytic bacteria recovered from the Populus euphratica at the disused (122 years ago) ancient Ugan river of middle reachs of Tarim river, and to understand their Phylogenetic diversity and community structure.
METHODS
Bacteria were isolated from the storage liquid in the stem of 2 Populus euphratica stands by using 3 types of different cultural medium (Luria-Bertani, Trypticase Soy Agar and Nutrient Agar), followed carry out 16S rDNA identifications and analysis of their biodiversity.
RESULTS
A total of 62 phenotypically different isolates were sequenced and according to their 16S rDNA sequence similarities to type strains of described organisms, they have been placed into four phylogenetic groups (1, Firmicutes; 2, Actinobacteria; 3, Alpha Proteobacteria and 4, Gamma Proteobacteria), 18 genera and 32 species. Among them, Bacillus and pseudomonas were the most widely distributed and predominant, occupied the majority of isolates 40.32% and 16.13%, respectively. Isolate KTH-63 (HM371419) formed a distinct clade with Macrococcus brunensis in phylogenetic tree based on 16S rDNA sequence among the family Staphylococcaceae, so it was demonstrated that the KTH-63 represents a potential novel genus and novel species within the family Staphylococcaceae with 92.491% sequence similarity with the described species Macrococcus brunensis of this family. Isolates KLH-1, KLH-21, KLH-18, KLH-25, KNA-3, KTH-8, KTH-14, KTH-20 and KNA-26 with 96.089% - 97.769% sequence similarities to their closely related members were presumed to be potential novel species, and the discovery rate of potential novel species in the endophytic bacterial community of Populus euphratica was reach up to 16.13%. Furthermore, 10 genera and 18 species have been added to the plant endophytic bacterial categoria by the data obtained in this work.
CONCLUSION
The result showed that the cultivable endophytic bacterial diversity in Populus euphratica at Ugan river was very abundant and have high percentage of potential novel species, and it have greatly refreshed the plant endophytic bacterial records. The community structure obtained in this study also may be presumed as a miniature of the endophytic bacterial flora in the Populus euphratica during the recent ages before the affect of modern civilization prossessing to Tarim river valley, which deserve further study and exploitation.
PubMed: 21574378
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].</title><author><name sortKey="Yusuf, Khayir" sort="Yusuf, Khayir" uniqKey="Yusuf K" first="Khayir" last="Yusuf">Khayir Yusuf</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. abdukaharyusuf@163.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Xinjiang University, Urumqi 830046</wicri:regionArea><wicri:noRegion>Urumqi 830046</wicri:noRegion></affiliation></author><author><name sortKey="Pulat, Shirenay" sort="Pulat, Shirenay" uniqKey="Pulat S" first="Shirenay" last="Pulat">Shirenay Pulat</name></author><author><name sortKey="Turdahun, Maripat" sort="Turdahun, Maripat" uniqKey="Turdahun M" first="Maripat" last="Turdahun">Maripat Turdahun</name></author><author><name sortKey="Hamdun, Maryam" sort="Hamdun, Maryam" uniqKey="Hamdun M" first="Maryam" last="Hamdun">Maryam Hamdun</name></author><author><name sortKey="Osman, Ghenijan" sort="Osman, Ghenijan" uniqKey="Osman G" first="Ghenijan" last="Osman">Ghenijan Osman</name></author><author><name sortKey="Abdurehim, Zumrat" sort="Abdurehim, Zumrat" uniqKey="Abdurehim Z" first="Zumrat" last="Abdurehim">Zumrat Abdurehim</name></author><author><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21574378</idno><idno type="pmid">21574378</idno><idno type="wicri:Area/Main/Corpus">002E13</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002E13</idno><idno type="wicri:Area/Main/Curation">002E13</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002E13</idno><idno type="wicri:Area/Main/Exploration">002E13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].</title><author><name sortKey="Yusuf, Khayir" sort="Yusuf, Khayir" uniqKey="Yusuf K" first="Khayir" last="Yusuf">Khayir Yusuf</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. abdukaharyusuf@163.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science and Technology, Xinjiang University, Urumqi 830046</wicri:regionArea><wicri:noRegion>Urumqi 830046</wicri:noRegion></affiliation></author><author><name sortKey="Pulat, Shirenay" sort="Pulat, Shirenay" uniqKey="Pulat S" first="Shirenay" last="Pulat">Shirenay Pulat</name></author><author><name sortKey="Turdahun, Maripat" sort="Turdahun, Maripat" uniqKey="Turdahun M" first="Maripat" last="Turdahun">Maripat Turdahun</name></author><author><name sortKey="Hamdun, Maryam" sort="Hamdun, Maryam" uniqKey="Hamdun M" first="Maryam" last="Hamdun">Maryam Hamdun</name></author><author><name sortKey="Osman, Ghenijan" sort="Osman, Ghenijan" uniqKey="Osman G" first="Ghenijan" last="Osman">Ghenijan Osman</name></author><author><name sortKey="Abdurehim, Zumrat" sort="Abdurehim, Zumrat" uniqKey="Abdurehim Z" first="Zumrat" last="Abdurehim">Zumrat Abdurehim</name></author><author><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta microbiologica Sinica</title><idno type="ISSN">0001-6209</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (classification)</term><term>Bacteria (genetics)</term><term>Bacteria (isolation & purification)</term><term>Bacterial Physiological Phenomena (MeSH)</term><term>Biodiversity (MeSH)</term><term>China (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Populus (microbiology)</term><term>Populus (physiology)</term><term>Rivers (microbiology)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bactéries (classification)</term><term>Bactéries (génétique)</term><term>Bactéries (isolement et purification)</term><term>Biodiversité (MeSH)</term><term>Chine (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Phénomènes physiologiques bactériens (MeSH)</term><term>Populus (microbiologie)</term><term>Populus (physiologie)</term><term>Rivières (microbiologie)</term><term>Symbiose (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term><term>Rivières</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term><term>Rivers</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacterial Physiological Phenomena</term><term>Biodiversity</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bactéries</term><term>Biodiversité</term><term>Chine</term><term>Données de séquences moléculaires</term><term>Phylogenèse</term><term>Phénomènes physiologiques bactériens</term><term>Symbiose</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>The aim of this study was to identify the culturable endophytic bacteria recovered from the Populus euphratica at the disused (122 years ago) ancient Ugan river of middle reachs of Tarim river, and to understand their Phylogenetic diversity and community structure.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Bacteria were isolated from the storage liquid in the stem of 2 Populus euphratica stands by using 3 types of different cultural medium (Luria-Bertani, Trypticase Soy Agar and Nutrient Agar), followed carry out 16S rDNA identifications and analysis of their biodiversity.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>A total of 62 phenotypically different isolates were sequenced and according to their 16S rDNA sequence similarities to type strains of described organisms, they have been placed into four phylogenetic groups (1, Firmicutes; 2, Actinobacteria; 3, Alpha Proteobacteria and 4, Gamma Proteobacteria), 18 genera and 32 species. Among them, Bacillus and pseudomonas were the most widely distributed and predominant, occupied the majority of isolates 40.32% and 16.13%, respectively. Isolate KTH-63 (HM371419) formed a distinct clade with Macrococcus brunensis in phylogenetic tree based on 16S rDNA sequence among the family Staphylococcaceae, so it was demonstrated that the KTH-63 represents a potential novel genus and novel species within the family Staphylococcaceae with 92.491% sequence similarity with the described species Macrococcus brunensis of this family. Isolates KLH-1, KLH-21, KLH-18, KLH-25, KNA-3, KTH-8, KTH-14, KTH-20 and KNA-26 with 96.089% - 97.769% sequence similarities to their closely related members were presumed to be potential novel species, and the discovery rate of potential novel species in the endophytic bacterial community of Populus euphratica was reach up to 16.13%. Furthermore, 10 genera and 18 species have been added to the plant endophytic bacterial categoria by the data obtained in this work.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The result showed that the cultivable endophytic bacterial diversity in Populus euphratica at Ugan river was very abundant and have high percentage of potential novel species, and it have greatly refreshed the plant endophytic bacterial records. The community structure obtained in this study also may be presumed as a miniature of the endophytic bacterial flora in the Populus euphratica during the recent ages before the affect of modern civilization prossessing to Tarim river valley, which deserve further study and exploitation.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21574378</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2012</Year><Month>04</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>51</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].</ArticleTitle><Pagination><MedlinePgn>178-88</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The aim of this study was to identify the culturable endophytic bacteria recovered from the Populus euphratica at the disused (122 years ago) ancient Ugan river of middle reachs of Tarim river, and to understand their Phylogenetic diversity and community structure.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Bacteria were isolated from the storage liquid in the stem of 2 Populus euphratica stands by using 3 types of different cultural medium (Luria-Bertani, Trypticase Soy Agar and Nutrient Agar), followed carry out 16S rDNA identifications and analysis of their biodiversity.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">A total of 62 phenotypically different isolates were sequenced and according to their 16S rDNA sequence similarities to type strains of described organisms, they have been placed into four phylogenetic groups (1, Firmicutes; 2, Actinobacteria; 3, Alpha Proteobacteria and 4, Gamma Proteobacteria), 18 genera and 32 species. Among them, Bacillus and pseudomonas were the most widely distributed and predominant, occupied the majority of isolates 40.32% and 16.13%, respectively. Isolate KTH-63 (HM371419) formed a distinct clade with Macrococcus brunensis in phylogenetic tree based on 16S rDNA sequence among the family Staphylococcaceae, so it was demonstrated that the KTH-63 represents a potential novel genus and novel species within the family Staphylococcaceae with 92.491% sequence similarity with the described species Macrococcus brunensis of this family. Isolates KLH-1, KLH-21, KLH-18, KLH-25, KNA-3, KTH-8, KTH-14, KTH-20 and KNA-26 with 96.089% - 97.769% sequence similarities to their closely related members were presumed to be potential novel species, and the discovery rate of potential novel species in the endophytic bacterial community of Populus euphratica was reach up to 16.13%. Furthermore, 10 genera and 18 species have been added to the plant endophytic bacterial categoria by the data obtained in this work.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The result showed that the cultivable endophytic bacterial diversity in Populus euphratica at Ugan river was very abundant and have high percentage of potential novel species, and it have greatly refreshed the plant endophytic bacterial records. The community structure obtained in this study also may be presumed as a miniature of the endophytic bacterial flora in the Populus euphratica during the recent ages before the affect of modern civilization prossessing to Tarim river valley, which deserve further study and exploitation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yusuf</LastName><ForeName>Khayir</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. abdukaharyusuf@163.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pulat</LastName><ForeName>Shirenay</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Turdahun</LastName><ForeName>Maripat</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Hamdun</LastName><ForeName>Maryam</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Osman</LastName><ForeName>Ghenijan</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Abdurehim</LastName><ForeName>Zumrat</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Rahman</LastName><ForeName>Erkin</ForeName><Initials>E</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018407" MajorTopicYN="N">Bacterial Physiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="N">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045483" MajorTopicYN="N">Rivers</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21574378</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Abdurehim, Zumrat" sort="Abdurehim, Zumrat" uniqKey="Abdurehim Z" first="Zumrat" last="Abdurehim">Zumrat Abdurehim</name><name sortKey="Hamdun, Maryam" sort="Hamdun, Maryam" uniqKey="Hamdun M" first="Maryam" last="Hamdun">Maryam Hamdun</name><name sortKey="Osman, Ghenijan" sort="Osman, Ghenijan" uniqKey="Osman G" first="Ghenijan" last="Osman">Ghenijan Osman</name><name sortKey="Pulat, Shirenay" sort="Pulat, Shirenay" uniqKey="Pulat S" first="Shirenay" last="Pulat">Shirenay Pulat</name><name sortKey="Rahman, Erkin" sort="Rahman, Erkin" uniqKey="Rahman E" first="Erkin" last="Rahman">Erkin Rahman</name><name sortKey="Turdahun, Maripat" sort="Turdahun, Maripat" uniqKey="Turdahun M" first="Maripat" last="Turdahun">Maripat Turdahun</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yusuf, Khayir" sort="Yusuf, Khayir" uniqKey="Yusuf K" first="Khayir" last="Yusuf">Khayir Yusuf</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002C40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21574378
|texte= [Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21574378" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |